Deleted
Sample Discontinued
Experience: None
No Sequence
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Part:BBa_J42022:Design

Designed by: Steve Selinsky   Group: iGEM2006_Michigan   (2006-10-30)


PolyLinker for Nested Landing Pad


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Polylinker had to be set up in a manner to facilitate compatible adjacent double digests. Also, polylinker sites had to be completely unique to vector sequence.


Source

Plasmid comes from pBR322, the inserted polylinkers was a custom construct of two annealed oligonucleotides from invitrogen.

References